Application

Research Sub CategoryIntegrins

Anti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.

Research CategoryCell Structure

Flow cytometry: 2 µg per 10⁶ cells

Immunohistochemistry on frozen tissue sections

Immunoprecipitation

Cell adhesion assays

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Virtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1131

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.

Format: Purified

Specificity

Reactivity with other species has not been confirmed.

Clone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.

Storage and Stability

Maintain at -20°C for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.